HPLC-ELSD检测方法对日本米酒的检测分析

HPLC condition

Sample: Japanese Sake

Column:Kromasil C18,5u,4.6×100mm

Mobile phase; 20mM NFPA, 20mM NFPA containing 70 % CH3CN Gradient

Flow rate: 0.8 ml/min

SofTA ELSD:  Spray Chamber 15°C Drift Tube 50°C, FLT=6, Gain=Normal,

5VFS

UV: 254 nm & 210 nm