HPLC-ELSD检测方法对氨基酸检测分析

HPLC condition

Column: 琛航色谱柱91181545  C18，5u，4.6 x 150 mm

1:Gly, 2: Ser 3: Ala, 4: Thr, 5: Asn, 6: Pro,

7: Asp 8: Gaba, 9: Gln, 10: E, 11: V, 12: Lys,

13: Leu, 14: Cys, 15: Met, 16: Ile, 17: His,

18: Arg, 19: Phe, 20: Tyr, 21: Impurity, 22: Trp

SofTA ELSD ：Spray Chamber: 30°C

                           Drift Tube: 55°C

Mobile Phase: A: Nonafluoropentanoic acid

                           B: ACN/ Nonafluoropentanoic acid

Sample: 10nM Each Amino Acid
 

Peaks

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